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Ionic strength and solvent dielectric dependence of K1d
Figure 2.
Effect of ionic strength on the first step equilibrium constant, K1d, for binding of camphor to wild-type cytochrome P450cam (open circles) and the D251N mutant (closed circles). Experiments were performed at 20 deg. C. in 100mM Tris-HCl at pH 7. [2].
Figure 3.
Effect of altering the solvent dielectric constant on the first step equilibrium constant, K1d, for the binding of camphor to wild-type cytochrome P450cam (open circles) and the D251N mutant (closed circles). Experiments were performed at 20 deg. C. in a water/ethylene glycol mixture at pH 7. Addition of increasing amounts of salt to a 50% ethylene glycol-containing sample showed that the effect on K1d induced by the dielectric constant could be reversed by changes in the ionic strength [2].
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Wade et al. (1996) Fundamentals of Enzyme-Ligand Interactions in
Cytochrome P450cam
POPE5 Conference Proceedings
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