GENERAL

Documentation on ww domains (also known as rsp5 / WWP domains)

Species Distribution

WW domain containing proteins in the yeast genome

Protein	links	SacchDB (systematic name)	SwissPROT (accession number)	Protein name	Gene name	Function	other domains (SMART)
Q06525	SwissProt SGD MIPS	YPR152C	Q06525	`SIMILAR TO S. CEREVISIAE HYPOTHETICAL PROTEIN YKL012P`	YPR152C or P9659.21	unknown	FF domain often accompanies WW domains. named after two conserved Phe. May be involved in protein-protein interactions.
ESS1	SwissProt SGD MIPS	YJR017C	P22696	ESS1	ESS1 or PTF1 or YJR017C or J1452	peptidyl-prolyl isomerase, mRNA processing, Mitotic regulator; structurally and functionally homologous to human PIN1	-
PR40	SwissProt SGD MIPS	YKL012W	P33203	Pre-mRNA processing protein PRP40	PRP40 or YKL012W or YKL165	`REQUIRED FOR THE FIRST STEP OF PRE-MRNA SPLICING. THIS PROTEIN IS ASSOCIATED WITH SNRNP U1.`	4 FF domains often accompanies WW domains. named after two conserved Phe. May be involved in protein-protein interactions.
RSP5	SwissProt SGD MIPS	YER125W	P39940	Ubiquitin--protein ligase RSP5	RSP5 or NPI1 or MDP1 or YER125W or SYGP-ORF41	`E3 UBIQUITIN-PROTEIN LIGASE` `REQUIRED FOR DEGRADATION OF SEVERAL CELL SURFACE PROTEINS LIKE GAP1, FUR4, MAL61 AND STE2. ALSO ACTS ON RBP1.`	1 C2 domain Ca2+ binding motif present in phospholipases, protein kinases and synaptotamins. Particular C2 appears to bind phospholipids, inositol phosphates and intracellular proteins. 1 Hect domain E3 ubiquitin-protein ligases. Can bind to E2 enzymes.
YFB0	SwissProt SGD MIPS	YFL010C	P43582	Hypothetical 22.7 kDa protein in AUA1-CDC4 intergenic region	YFL010C	unknown protein interacting with Metacaspase (MCA1)	-
SET2/YJQ8	SwissProt SGD MIPS	YJL168C	P46995	Hypothetical 84.5 kDa protein in CPS1-FPP1 intergenic region	YJL168C or J0520	unknown systematic deletion viable	AWS domain subdomain of preset. Associated with SET domain. SET domain Putative methyl transferase, based on outlier plant homologues PostSet domain Cys-rich motif following a subset of SET domains.
TIN/YGX7_YEAST		YGL227W	P53076	Hypothetical 108.2 kDa protein in SAP4-OST5 intergenic region	YGL227W	molecular function unknown important for vacuole import and degradation (VID); TOR inhibitor (TIN)
SSM4		YIL030C	P40318	SSM4	SSM4 or YIL030C or YI3299.01C or YI9905.18C	molecular function unknown Protein involved in mRNA turnover (polyadenylation)

Protein	domains	motif recognized by the other domains
Q06525	FF domain	FF domain in CA150 (transcription factor, negative regulator of RNAPII) binds the phosphorylated C terminal repeat of RNA polymerase II (CTD). CTD contains 52 YSPTSPS repeats. [1]
PRP40	4 FF domain	FF domain in CA150 binds the phosphorylated C terminal repeat of RNA polymerase II (CTD). CTD contains 52 YSPTSPS repeats. [1]
RSP5	C2 domain	generally binds membrane regions
	HECT domain	various HECT domains bind different E2 enzymes, not clear if there is any consensus binding sequence
SET2/YJQ8	AWS / Postset domains	No specif litterature
	SET domain	This domain was originally discoverd in Drosophila proteins Su(var)3-9 [2]. Most of the SET domain-containing proteins that have been characterized thus far are associated with chromatin [3], suggesting that these proteins are involved in chromatin regulation and chromossome function. However not all SET domains appear to have the same function. For example, the human and mouse SUV39H1, SUV38H2 (homologues of Su(var)3-9) have histone H3 methyltransferase (HMTase) activity on lysine 9 [4,5]. CLR4, the corresponding yeast homologue, also displays HMTase activity [4]. A catalytic motif in the SET domain and two cystein-rich regions flanking the SET domain appear to play an important role in this activity [4]. Conversely, the human SET domain containing proteins EZH2 and HRX, and their Drosophila homologues TRX and E(Z), do not display any HMTase activity in vitro [4] and appear instead to modulate protein-protein interactions [6]. All of these proteins lack one or more of the cystein-rich flanking regions. Another recently discovered protein with HMTase activity, both on Lys 9 and lys 27, is the human G9a protein [7]. Like the SUV39H family, G9a contains the SET domain as well as the PreSet and PostSet domains. Analysis of the S. cerevisae genome database identified six SET domain-containing proteins, yet none of these proteins contains both the PreSET and PostSET domains (see SMART). Only Set1 and Set2 proteins have the postSET domain. It has recently been shown that SET1 has HMTase activity on Lys 4 [8], rather than on Lys 9, and that SET2, has HMTase activity on lys 36 [9]. Consistent with the idea that the function of the adjacent cystein-rich flanking domains might be replaced by other motifs or other proteins [10], a novel H3 HMTase protein (human set9) was recently discovered that lacks both PreSET and postSET domain [11]

References (besides SMART litterature):

[1] D. P. Morris & A. L. Greenleaf (2000) JBC, 275, 39935 (medline ); A.C. Goldstrohm et al. (2001)Mol. Cell. Biol. 21, 7617 (medline ); M.C. Carty et al. (2000) PNAS, 97, 9015.
[2] B. Tschiersch (1994) EMBO J, 13, 3822.
[3] B. Kuzin et al. (1994) Genes Dev., 8, 2478 (medline ); S Petruk et al. (2001) Science, 294, 1331(medline ); T Rozovskaia et al. (2000), Oncogene, 19, 351 (medline ); T Rozovskaia et al. (1999), Mol Cell Biol, 19, 6441 (medline ); JP Vielle-Calzada et al (1999), Genes Dev, 13, 2971 (medline ); L Xu, S Strome (2001), Genetics, 159, 1019 (medline); L Xu et al (2001) PNAS, 98, 5061-6 (medline)
[4] S. Rea et al. (2000) Nature, 406, 593-9 (medline)
[5] AH. Peters et al (2002), Nat Gen, 30, 77 (medline)
[6] XI Cui et al (1998) Nat. Genet., 18, 331(medline); T Rozovskaia et al. (1999), Mol Cell Biol, 19, 6441 (medline )
[7] M Tachibana et al (2001) JBC, 20, 20 (medline)
[8] PL Nagy et al (2002), PNAS, 99, 90 (medline)
[9] BD Strahl et al (2002) Mol Cel Biol, 22, 1298 (medline)
[10] Y Zhang & D. Reinberg (2001) Genes Dev, 15, 2343 (medline) review
[11] K. Nishioka et al (1999) Genes Dev, 16, 479 medline

Protein Sequence

Q06525 >tr|Q06525 SIMILAR TO S. CEREVISIAE HYPOTHETICAL PROTEIN YKL012P - Saccharomyces cerevisiae (Baker's yeast).
MRGEWQEFKTPAGKKYYYNKNTKQSRWEKPNLKKGSNLESNAKESQTERKPTFSLELVNG
WHLIIYNDGTKLYFNDDSKEFKNDISQEDDSRCRSLIESLDKEKLVLLIGVARGYTMREE
DIDKILESCNEEIHLFKRNQDEVERKDEISEEAGDVKSPLQESHTGLVSGYGSSSGEEDE
EEDEEEDEENEEQIVNQDISIIDDLNRIDTDDIDERNIFFELFDRYKLDKFSTWSLQSKK
IENDPDFYKIRDDTVRESLFEEWCGERSGNATAEESDSEDNSEDDSEVLEPTKYHYLAQI
VANAGTIAPDTIPQDIRKQQKALYKAYKIKEYIPSKRDQDKFVSQLLFYYKTFDLEQRKE
IFCDCLRDHERDFTGAVESLRQDKELIDRWQTLLKAPADSSSIEDILLSIEHRCCVSPIV
VTEPRYYVVGILEKTVVWVRWLAAEVGPSSRFTPVGAGNEPINPE

ESS1_YEAST >sp|P22696|ESS1_YEAST ESS1 protein (Processing/termination factor 1) - Saccharomyces cerevisiae (Baker's yeast).
MIIVVLIPTHNRLRHHNDAPMPSDVASRTGLPTPWTVRYSKSKKREYFFNPETKHSQWEE
PEGTNKDQLHKHLRDHPVRVRCLHILIKHKDSRRPASHRSENITISKQDATDELKTLITR
LDDDSKTNSFEALAKERSDCSSYKRGGDLGWFGRGEMQPSFEDAAFQLKVGEVSDIVESG
SGVHVIKR

PR40_YEAST >sp|P33203|PR40_YEAST Pre-mRNA processing protein PRP40 - Saccharomyces cerevisiae (Baker's yeast).
MSIWKEAKDASGRIYYYNTLTKKSTWEKPKELISQEELLLRENGWKAAKTADGKVYYYNP
TTRETSWTIPAFEKKVEPIAEQKHDTVSHAQVNGNRIALTAGEKQEPGRTINEEESQYAN
NSKLLNVRRRTKEEAEKEFITMLKENQVDSTWSFSRIISELGTRDPRYWMVDDDPLWKKE
MFEKYLSNRSADQLLKEHNETSKFKEAFQKMLQNNSHIKYYTRWPTAKRLIADEPIYKHS
VVNEKTKRQTFQDYIDTLIDTQKESKKKLKTQALKELREYLNGIITTSSSETFITWQQLL
NHYVFDKSKRYMANRHFKVLTHEDVLNEYLKIVNTIENDLQNKLNELRLRNYTRDRIARD
NFKSLLREVPIKIKANTRWSDIYPHIKSDPRFLHMLGRNGSSCLDLFLDFVDEQRMYIFA
QRSIAQQTLIDQNFEWNDADSDEITKQNIEKVLENDRKFDKVDKEDISLIVDGLIKQRNE
KIQQKLQNERRILEQKKHYFWLLLQRTYTKTGKPKPSTWDLASKELGESLEYKALGDEDN
IRRQIFEDFKPESSAPTAESATANLTLTASKKRHLTPAVELDY

RSP5_YEAST >sp|P39940|RSP5_YEAST Ubiquitin--protein ligase RSP5 (EC 6.3.2.-) - Saccharomyces cerevisiae (Baker's yeast).
MPSSISVKLVAAESLYKRDVFRSPDPFAVLTIDGYQTKSTSAAKKTLNPYWNETFKFDDI
NENSILTIQVFDQKKFKKKDQGFLGVVNVRVGDVLGHLDEDTATSSGRPREETITRDLKK
SNDGMAVSGRLIVVLSKLPSSSPHSQAPSGHTASSSTNTSSTTRTNGHSTSSTRNHSTSH
PSRGTAQAVESTLQSGTTAATNTATTSHRSTNSTSSATRQYSSFEDQYGRLPPGWERRTD
NFGRTYYVDHNTRTTTWKRPTLDQTEAERGNQLNANTELERRQHRGRTLPGGSSDNSSVT
VQVGGGSNIPPVNGAAAAAFAATGGTTSGLGELPSGWEQRFTPEGRAYFVDHNTRTTTWV
DPRRQQYIRTYGPTNTTIQQQPVSQLGPLPSGWEMRLTNTARVYFVDHNTKTTTWDDPRL
PSSLDQNVPQYKRDFRRKVIYFRSQPALRILPGQCHIKVRRKNIFEDAYQEIMRQTPEDL
KKRLMIKFDGEEGLDYGGVSREFFFLLSHEMFNPFYCLFEYSAYDNYTIQINPNSGINPE
HLNYFKFIGRVVGLGVFHRRFLDAFFVGALYKMMLRKKVVLQDMEGVDAEVYNSLNWMLE
NSIDGVLDLTFSADDERFGEVVTVDLKPDGRNIEVTDGNKKEYVELYTQWRIVDRVQEQF
KAFMDGFNELIPEDLVTVFDERELELLIGGIAEIDIEDWKKHTDYRGYQESDEVIQWFWK
CVSEWDNEQRARLLQFTTGTSRIPVNGFKDLQGSDGPRRFTIEKAGEVQQLPKSHTCFNR
VDLPQYVDYDSMKQKLTLAVEETIGFGQE

YFB0_YEAST >sp|P43582|YFB0_YEAST Hypothetical 22.7 kDa protein in AUA1-CDC4 intergenic region - Saccharomyces cerevisiae (Baker's yeast).
MAQSKSNPPQVPSGWKAVFDDEYQTWYYVDLSTNSSQWEPPRGTTWPRPKGPPPGVNNEK
SSRQQADQAPPPYSSQSTPQVQAGAQAQQPRYYQPQQPQYPQYPQQQRYYPQQAPMPAAA
PQQAYYGTAPSTSKGSGHGGAMMGGLLGVGAGLLGGAMLEHAFDDHNYDGPDTVVVENNY
YGDDAGGSDGGFDDAGGFDGGFDDGFDGSDF

YJQ8_YEAST >sp|P46995|YJQ8_YEAST Hypothetical 84.5 kDa protein in CPS1-FPP1 intergenic region - Saccharomyces cerevisiae (Baker's yeast).
MSKNQSVSASEDEKEILNNNAEGHKPQRLFDQEPDLTEEALTKFENLDDCIYANKRIGTF
KNNDFMECDCYEEFSDGVNHACDEDSDCINRLTLIECVNDLCSSCGNDCQNQRFQKKQYA
PIAIFKTKHKGYGVRAEQDIEANQFIYEYKGEVIEEMEFRDRLIDYDQRHFKHFYFMMLQ
NGEFIDATIKGSLARFCNHSCSPNAYVNKWVVKDKLRMGIFAQRKILKGEEITFDYNVDR
YGAQAQKCYCEEPNCIGFLGGKTQTDAASLLPQNIADALGVTVSMEKKWLKLKKLSGEPI
IKNENENINIEFLQSLEVQPIDSPVDVTKIMSVLLQQDNKIIASKLLKRLFTIDDDSLRH
QAIKLHGYTCFSKMLKLFITEQPQVDGKGNETEEDDIKFIKGILDFLLELPKTTRNGIES
SQIDNVVKTLPAKFPFLKPNCDELLEKWSKFETYKRITKKDINVAASKMIDLRRVRLPPG
WEIIHENGRPLYYNAEQKTKLHYPPSGSSKVFSSRSNTQVNSPSSSGIPKTPGALDSKKH
KLSDEEYERKKQKRLEYERIALERAKQEELESLKQKLKLENERKSVLEDIIAEFNKQKEL
QKEESKKLVEAKEAKRLKRKTVSQSQRLEHNWNKFFASFVPNLIKKNPQSKQFDHENIKQ
CAKDIVKILTTKELKKDSSRAPPDDLTKGKRHKVKEFINSYMDKIILKKKQKKALGLSSA
STRMSSPPPSTSS

Privacy Imprint

Protein	Sequence
Q06525	`>tr\|Q06525 SIMILAR TO S. CEREVISIAE HYPOTHETICAL PROTEIN YKL012P - Saccharomyces cerevisiae (Baker's yeast).` `MRGEWQEFKTPAGKKYYYNKNTKQSRWEKPNLKKGSNLESNAKESQTERKPTFSLELVNG` `WHLIIYNDGTKLYFNDDSKEFKNDISQEDDSRCRSLIESLDKEKLVLLIGVARGYTMREE` `DIDKILESCNEEIHLFKRNQDEVERKDEISEEAGDVKSPLQESHTGLVSGYGSSSGEEDE` `EEDEEEDEENEEQIVNQDISIIDDLNRIDTDDIDERNIFFELFDRYKLDKFSTWSLQSKK` `IENDPDFYKIRDDTVRESLFEEWCGERSGNATAEESDSEDNSEDDSEVLEPTKYHYLAQI` `VANAGTIAPDTIPQDIRKQQKALYKAYKIKEYIPSKRDQDKFVSQLLFYYKTFDLEQRKE` `IFCDCLRDHERDFTGAVESLRQDKELIDRWQTLLKAPADSSSIEDILLSIEHRCCVSPIV` `VTEPRYYVVGILEKTVVWVRWLAAEVGPSSRFTPVGAGNEPINPE`
ESS1_YEAST	`>sp\|P22696\|ESS1_YEAST ESS1 protein (Processing/termination factor 1) - Saccharomyces cerevisiae (Baker's yeast).` `MIIVVLIPTHNRLRHHNDAPMPSDVASRTGLPTPWTVRYSKSKKREYFFNPETKHSQWEE` `PEGTNKDQLHKHLRDHPVRVRCLHILIKHKDSRRPASHRSENITISKQDATDELKTLITR` `LDDDSKTNSFEALAKERSDCSSYKRGGDLGWFGRGEMQPSFEDAAFQLKVGEVSDIVESG` `SGVHVIKR`
PR40_YEAST	`>sp\|P33203\|PR40_YEAST Pre-mRNA processing protein PRP40 - Saccharomyces cerevisiae (Baker's yeast).` `MSIWKEAKDASGRIYYYNTLTKKSTWEKPKELISQEELLLRENGWKAAKTADGKVYYYNP` `TTRETSWTIPAFEKKVEPIAEQKHDTVSHAQVNGNRIALTAGEKQEPGRTINEEESQYAN` `NSKLLNVRRRTKEEAEKEFITMLKENQVDSTWSFSRIISELGTRDPRYWMVDDDPLWKKE` `MFEKYLSNRSADQLLKEHNETSKFKEAFQKMLQNNSHIKYYTRWPTAKRLIADEPIYKHS` `VVNEKTKRQTFQDYIDTLIDTQKESKKKLKTQALKELREYLNGIITTSSSETFITWQQLL` `NHYVFDKSKRYMANRHFKVLTHEDVLNEYLKIVNTIENDLQNKLNELRLRNYTRDRIARD` `NFKSLLREVPIKIKANTRWSDIYPHIKSDPRFLHMLGRNGSSCLDLFLDFVDEQRMYIFA` `QRSIAQQTLIDQNFEWNDADSDEITKQNIEKVLENDRKFDKVDKEDISLIVDGLIKQRNE` `KIQQKLQNERRILEQKKHYFWLLLQRTYTKTGKPKPSTWDLASKELGESLEYKALGDEDN` `IRRQIFEDFKPESSAPTAESATANLTLTASKKRHLTPAVELDY`
RSP5_YEAST	`>sp\|P39940\|RSP5_YEAST Ubiquitin--protein ligase RSP5 (EC 6.3.2.-) - Saccharomyces cerevisiae (Baker's yeast).` `MPSSISVKLVAAESLYKRDVFRSPDPFAVLTIDGYQTKSTSAAKKTLNPYWNETFKFDDI` `NENSILTIQVFDQKKFKKKDQGFLGVVNVRVGDVLGHLDEDTATSSGRPREETITRDLKK` `SNDGMAVSGRLIVVLSKLPSSSPHSQAPSGHTASSSTNTSSTTRTNGHSTSSTRNHSTSH` `PSRGTAQAVESTLQSGTTAATNTATTSHRSTNSTSSATRQYSSFEDQYGRLPPGWERRTD` `NFGRTYYVDHNTRTTTWKRPTLDQTEAERGNQLNANTELERRQHRGRTLPGGSSDNSSVT` `VQVGGGSNIPPVNGAAAAAFAATGGTTSGLGELPSGWEQRFTPEGRAYFVDHNTRTTTWV` `DPRRQQYIRTYGPTNTTIQQQPVSQLGPLPSGWEMRLTNTARVYFVDHNTKTTTWDDPRL` `PSSLDQNVPQYKRDFRRKVIYFRSQPALRILPGQCHIKVRRKNIFEDAYQEIMRQTPEDL` `KKRLMIKFDGEEGLDYGGVSREFFFLLSHEMFNPFYCLFEYSAYDNYTIQINPNSGINPE` `HLNYFKFIGRVVGLGVFHRRFLDAFFVGALYKMMLRKKVVLQDMEGVDAEVYNSLNWMLE` `NSIDGVLDLTFSADDERFGEVVTVDLKPDGRNIEVTDGNKKEYVELYTQWRIVDRVQEQF` `KAFMDGFNELIPEDLVTVFDERELELLIGGIAEIDIEDWKKHTDYRGYQESDEVIQWFWK` `CVSEWDNEQRARLLQFTTGTSRIPVNGFKDLQGSDGPRRFTIEKAGEVQQLPKSHTCFNR` `VDLPQYVDYDSMKQKLTLAVEETIGFGQE`
YFB0_YEAST	`>sp\|P43582\|YFB0_YEAST Hypothetical 22.7 kDa protein in AUA1-CDC4 intergenic region - Saccharomyces cerevisiae (Baker's yeast).` `MAQSKSNPPQVPSGWKAVFDDEYQTWYYVDLSTNSSQWEPPRGTTWPRPKGPPPGVNNEK` `SSRQQADQAPPPYSSQSTPQVQAGAQAQQPRYYQPQQPQYPQYPQQQRYYPQQAPMPAAA` `PQQAYYGTAPSTSKGSGHGGAMMGGLLGVGAGLLGGAMLEHAFDDHNYDGPDTVVVENNY` `YGDDAGGSDGGFDDAGGFDGGFDDGFDGSDF`
YJQ8_YEAST	`>sp\|P46995\|YJQ8_YEAST Hypothetical 84.5 kDa protein in CPS1-FPP1 intergenic region - Saccharomyces cerevisiae (Baker's yeast).` `MSKNQSVSASEDEKEILNNNAEGHKPQRLFDQEPDLTEEALTKFENLDDCIYANKRIGTF` `KNNDFMECDCYEEFSDGVNHACDEDSDCINRLTLIECVNDLCSSCGNDCQNQRFQKKQYA` `PIAIFKTKHKGYGVRAEQDIEANQFIYEYKGEVIEEMEFRDRLIDYDQRHFKHFYFMMLQ` `NGEFIDATIKGSLARFCNHSCSPNAYVNKWVVKDKLRMGIFAQRKILKGEEITFDYNVDR` `YGAQAQKCYCEEPNCIGFLGGKTQTDAASLLPQNIADALGVTVSMEKKWLKLKKLSGEPI` `IKNENENINIEFLQSLEVQPIDSPVDVTKIMSVLLQQDNKIIASKLLKRLFTIDDDSLRH` `QAIKLHGYTCFSKMLKLFITEQPQVDGKGNETEEDDIKFIKGILDFLLELPKTTRNGIES` `SQIDNVVKTLPAKFPFLKPNCDELLEKWSKFETYKRITKKDINVAASKMIDLRRVRLPPG` `WEIIHENGRPLYYNAEQKTKLHYPPSGSSKVFSSRSNTQVNSPSSSGIPKTPGALDSKKH` `KLSDEEYERKKQKRLEYERIALERAKQEELESLKQKLKLENERKSVLEDIIAEFNKQKEL` `QKEESKKLVEAKEAKRLKRKTVSQSQRLEHNWNKFFASFVPNLIKKNPQSKQFDHENIKQ` `CAKDIVKILTTKELKKDSSRAPPDDLTKGKRHKVKEFINSYMDKIILKKKQKKALGLSSA` `STRMSSPPPSTSS`