• Yjl069c protein required for cell viability, function unknown
• Yor145c protein required for cell viability, function unknown
• Yml093w protein required for cell viability, function unknown
• Ylr409c protein required for cell viability, function unknown
• Ylr222c protein required for cell viability, function unknown
• Ykr096w Hypothetical ORF
• Ykr060w Hypothetical ORF
• Ykl099c protein required for cell viability, function unknown
• Yjl109c protein required for cell viability, function unknown
• Ygr090w protein required for cell viability, function unknown
• Ydr449c protein required for cell viability, function unknown
• Tfp1 Catalytic subunit (subunit A) of the vacuolar H(+) ATPase V1 complex, generated upon protein splicing of the full-length unspliced precursor protein by ligation of the N-terminal and C-terminal extein moieties (Lysosome/vacuole, Peripheral membrane)
• Sik1 Nucleolar protein component of box C/D snoRNPs, which are necessary for 2'-O-methylation of ribosomal RNAs (nuclear, nucleolus)
• Rrp5 Protein required for processing of pre-rRNA to 18S and 5.8S rRNA (nuclear, nucleolus)
• Pwp2 Essential protein required for cell separation (cytoplasmatic)
• Ptc5 Putative serine/threonine protein phosphatase of the PP2C family
• Prp40 U1 snRNA-associated protein required for pre-mRNA splicing
• Nop1 Fibrillarin, protein associated with small nucleolar RNA (snoRNA) and required for 35S rRNA processing and methylation (nuclear, nucleolus)
• Nan1 Nucleolar protein associated with Net1(Nucleolar protein required for rDNA chromatin silencing and nucleolar integrity) (nuclear, nucleolus)
• Lcp5 Protein required for ribosomal RNA processing (nuclear, nucleolus)
• Kre33 Killer toxin REsistant, Killer toxin REsistant, molecular function unknown
• Dip2 Dom34p-interacting protein, molecular function unknown
• Ckb2 Casein kinase II Protein kinase CK2, regulatory (beta-prime) subunit (nuclear)
• Ckb1 Casein kinase II (Protein kinase CK2), regulatory (beta) subunit (nuclear)
• Cka2 Casein kinase II (Protein kinase CK2), catalytic (alpha-prime) subunit
• Cka1 Casein kinase II (protein kinase CK2), catalytic (alpha) subunit

• cbc2 Small subunit of nuclear cap-binding protein complex (nuclear)
• mud1 U1 snRNP A protein (snRNA-associated protein) required for commitment complex formation during mRNA, contains two RNA recognition motif (RRM) domains.(nuclear)
• prp39 U1 snRNA-associated protein required for commitment of pre-mRNA to splicing pathway (nuclear)
• prp40 U1 snRNA-associated protein required for pre-mRNA splicing (nuclear)
• prp42 U1 snRNA-associated protein required for pre-mRNA splicing and U1 snRNP biogenesis (nuclear)
• snp1 U1 snRNA-associated protein with RNA recognition (RRM) domain (nuclear)
• snu56 U1 snRNA-associated protein (nuclear)
• snu71 U1 snRNA-associated protein (nuclear)
• sto1 Large subunit of the nuclear cap-binding protein complex (nuclear)

• cdc33 Translation initiation factor eIF4E, mRNA cap-binding protein found in association with Caf20
• hsh155 U2 snRNP protein, homolog of human SF3B1 U2 snRNP protein
• luc7 U1 snRNA-associated protein
• nam8 U1 snRNA-associated protein, essential for meiotic recombination, suppressor of mitochondrial splicing defects
• prp39 U1 snRNA-associated protein required for commitment of pre-mRNA to splicing pathway (nuclear)
• prp40 U1 snRNA-associated protein required for pre-mRNA splicing (nuclear)
• prp42 U1 snRNA-associated protein required for pre-mRNA splicing and U1 snRNP biogenesis (nuclear)
• rse1 U2 snRNP-associated protein involved in pre-mRNA splicing, indirectly involved in ER to Golgi transport (nuclear)
• smb1 Spliceosomal snRNA-associated Sm core protein, also likely associated with telomerase TLC1 RNA (nuclear)
• smd2 Spliceosomal snRNA-associated Sm core protein, also likely associated with telomerase TLC1 RNA (nuclear)
• smd3 Spliceosomal snRNA-associated Sm core protein required for pre-mRNA splicing, also associated with telomerase TLC1 RNA
• smx3 Spliceosomal snRNA-associated Sm core protein, also likely associated with telomerase TLC1 RNA (nuclear)
• snp1 U1 snRNA-associated protein with RNA recognition (RRM) domain (nuclear)
• snu56 U1 snRNA-associated protein (nuclear)
• snu71 U1 snRNA-associated protein (nuclear)
• sto1 Large subunit of the nuclear cap-binding protein complex (nuclear)
• snu114 Component of the U5 small nuclear ribonucleoprotein (U5 snRNP) required for pre-mRNA splicing (cytoplasmatic)
• tif4631 mRNA cap-binding protein (eIF4F) 150K subunit (nuclear)
• yhc1 U1 snRNP-specific C protein, component of the U1 snRNP that is needed for spliceosome assembly (nuclear)

• cbc2 Small subunit of nuclear cap-binding protein complex (nuclear)
• luc7 U1 snRNA-associated protein
• mud1 U1 snRNP A protein (snRNA-associated protein) required for commitment complex formation during mRNA, contains two RNA recognition motif (RRM) domains.(nuclear)
• nam8 U1 snRNA-associated protein, essential for meiotic recombination, suppressor of mitochondrial splicing defects (nuclear)
• npl3 Protein involved in 18S and 25S rRNA processing, export of RNA from the nucleus, import of proteins into the nucleus, associated with U1 snRNP (nuclear)
• prp11 Pre-mRNA splicing factor, associated with U2, U4, U5, and U6 snRNPs (nuclear)
• prp39 U1 snRNA-associated protein required for commitment of pre-mRNA to splicing pathway (nuclear)
• prp40 U1 snRNA-associated protein required for pre-mRNA splicing (nuclear)
• prp42 U1 snRNA-associated protein required for pre-mRNA splicing and U1 snRNP biogenesis (nuclear)
• smb1 Spliceosomal snRNA-associated Sm core protein, also likely associated with telomerase TLC1 RNA (nuclear)
• smd2 Spliceosomal snRNA-associated Sm core protein, also likely associated with telomerase TLC1 RNA (nuclear)
• smd3 Spliceosomal snRNA-associated Sm core protein required for pre-mRNA splicing, also associated with telomerase TLC1 RNA
• snp1 U1 snRNA-associated protein with RNA recognition (RRM) domain (nuclear)
• snu114 Component of the U5 small nuclear ribonucleoprotein (U5 snRNP) required for pre-mRNA splicing (cytoplasmatic)
• snu56 U1 snRNA-associated protein (nuclear)
• snu71 U1 snRNA-associated protein (nuclear)
• sto1 Large subunit of the nuclear cap-binding protein complex (nuclear)
• tif4631 mRNA cap-binding protein (eIF4F) 150K subunit (nuclear)
• yhc1 U1 snRNP-specific C protein, component of the U1 snRNP that is needed for spliceosome assembly (nuclear)
• yef3 Translation elongation factor EF-3A, member of the non-transporter group of the ATP-binding cassette (ABC) family (nuclear)

• brr1 Protein involved in snRNP biogenesis (nuclear)
• clf1 Essential pre-mRNA splicing factor (nuclear)
• cus1 U2 snRNP protein (nuclear)
• dib1 Component of the U4/U6.U5 snRNP, also required for mitotic spindle formation or function (nuclear)
• hsh155 U2 snRNP protein, homolog of human SF3B1 U2 snRNP protein (nuclear)
• lea1 Component of the U2 snRNP complex similar to human U2A' protein, involved in the initial steps of pre-mRNA splicing (nuclear)
• lsm4 U6 snRNA-associated protein of the Sm-like group (nuclear)
• luc7 U1 snRNA-associated protein (nuclear)
• mud1 U1 snRNP A protein (snRNA-associated protein) required for commitment complex formation during mRNA, contains two RNA recognition motif (RRM) domains.(nuclear)
• prp11 Pre-mRNA splicing factor, associated with U2, U4, U5, and U6 snRNPs (nuclear)
• prp19 Non-snRNP spliceosome component, also involved in mitotic recombination and gene conversion (nuclear)
• prp21 Pre-mRNA splicing factor (snRNA-associated protein), required for the addition of U2 snRNA to prespliceosomes (nuclear)
• prp3 Pre-mRNA splicing factor required for stability of U4/U6 snRNPs and for assembly of U4/U6.U5 triplex snRNP particle from U4/U6 and U5 snRNPs (nuclear)
• prp31 Protein required for pre-mRNA splicing, associated with U4/U6.U5 tri-snRNP (nuclear)
• prp4 U4/U6 snRNA-associated splicing factor (nuclear)
• prp40 U1 snRNA-associated protein required for pre-mRNA splicing (nuclear)
• prp46 Putative spliceosomal protein (nuclear)
• prp6 U4/U6.U5 tri-snRNP-associated splicing factor (nuclear)
• prp9 Pre-mRNA splicing factor (snRNA-associated protein), required for addition of U2 snRNA to prespliceosomes (nuclear)
• rse1 U2 snRNP-associated protein involved in pre-mRNA splicing, indirectly involved in ER to Golgi transport (nuclear)
• smd2 Spliceosomal snRNA-associated Sm core protein, also likely associated with telomerase TLC1 RNA (nuclear)
• sme1 Spliceosomal snRNA-associated Sm core protein required for mRNA splicing, also likely associated with telomerase TLC1 RNA (nuclear)
• snu114 Component of the U5 small nuclear ribonucleoprotein (U5 snRNP) required for pre-mRNA splicing (cytoplasmatic, RNA associated)
• srb2 Component of the RNA polymerase II holoenzyme and Kornberg's mediator (SRB) subcomplex (nuclear)

• cus1 U2 snRNP protein (nuclear)
• dib1 Component of the U4/U6.U5 snRNP, also required for mitotic spindle formation or function (nuclear)
• ecm2 Putative pre-mRNA splicing factor, also involved in cell wall structure or biosynthesis (nuclear)
• hsh155 U2 snRNP protein, homolog of human SF3B1 U2 snRNP protein (nuclear)
• lea1 Component of the U2 snRNP complex similar to human U2A' protein, involved in the initial steps of pre-mRNA splicing (nuclear)
• luc7 U1 snRNA-associated protein (nuclear)
• msl1 U2 snRNA-associated protein with similarity to U2B" protein (nuclear)
• prp19 Non-snRNP spliceosome component, also involved in mitotic recombination and gene conversion (nuclear)
• prp31 Protein required for pre-mRNA splicing, associated with U4/U6.U5 tri-snRNP (nuclear)
• prp39 U1 snRNA-associated protein required for commitment of pre-mRNA to splicing pathway (nuclear)
• prp4 U4/U6 snRNA-associated splicing factor (nuclear)
• prp40 U1 snRNA-associated protein required for pre-mRNA splicing (nuclear)
• prp42 U1 snRNA-associated protein required for pre-mRNA splicing and U1 snRNP biogenesis (nuclear)
• prp43 Pre-mRNA splicing factor, member of the DEAH-box RNA helicase family
• prp46 Putative spliceosomal protein (nuclear)
• prp6 U4/U6.U5 tri-snRNP-associated splicing factor (nuclear)
• prp9 Pre-mRNA splicing factor (snRNA-associated protein), required for addition of U2 snRNA to prespliceosomes (nuclear)
• rse1 U2 snRNP-associated protein involved in pre-mRNA splicing, indirectly involved in ER to Golgi transport (nuclear)
• smb1 Spliceosomal snRNA-associated Sm core protein, also likely associated with telomerase TLC1 RNA (nuclear)
• smd3 Spliceosomal snRNA-associated Sm core protein required for pre-mRNA splicing, also associated with telomerase TLC1 RNA (nuclear)
• smx3 Spliceosomal snRNA-associated Sm core protein, also likely associated with telomerase TLC1 RNA (nuclear)
• snt309 mRNA splicing factor, component of the Prp19p-associated complex and the spliceosome (nuclear)
• snu114 Component of the U5 small nuclear ribonucleoprotein (U5 snRNP) required for pre-mRNA splicing (cytoplasmatic, RNA associated)
• snu71 U1 snRNA-associated protein (nuclear)
• sto1 Large subunit of the nuclear cap-binding protein complex (nuclear)
• ydl209c molecular function unknown (nuclear)
• yjr084w molecular function unknown
• ylr424w molecular function unknown

• luc7 U1 snRNA-associated protein (nuclear)
• nam8 U1 snRNA-associated protein, essential for meiotic recombination, suppressor of mitochondrial splicing defects (nuclear)
• prp39 U1 snRNA-associated protein required for commitment of pre-mRNA to splicing pathway (nuclear)
• prp40 U1 snRNA-associated protein required for pre-mRNA splicing (nuclear)
• prp42 U1 snRNA-associated protein required for pre-mRNA splicing and U1 snRNP biogenesis (nuclear)
• snp1 U1 snRNA-associated protein with RNA recognition (RRM) domain (nuclear)
• snu56 U1 snRNA-associated protein (nuclear)
• snu71 U1 snRNA-associated protein (nuclear)
• sto1 Large subunit of the nuclear cap-binding protein complex (nuclear)

• brr1 Protein involved in snRNP biogenesis (nuclear)
• bur2 Divergent cyclin involved in regulation of transcription, causes chromosome loss when overproduced
• cbc2 Small subunit of nuclear cap-binding protein complex (nuclear)
• hta1 Histone H2A (nuclear)
• kap95 Karyopherin-beta, targets proteins with nuclear localization (NLS) sequences to the nuclear pore complex (nuclear, cytoplasmatic, nuclear pore)
• luc7 U1 snRNA-associated protein (nuclear)
• msh4 Meiosis-specific protein homologous to E. coli MutS (nuclear)
• mud1 U1 snRNP A protein (snRNA-associated protein) required for commitment complex formation during mRNA, contains two RNA recognition motif (RRM) domains.(nuclear)
• nab3 Nuclear polyadenylated RNA-binding protein (nuclear)
• nrd1 Protein that controls transcriptional elongation, interacts with the C-terminal domain of RNA polymerase II large subunit and binds DNA through a RNA recognition (RRM) domain
• prp40 U1 snRNA-associated protein required for pre-mRNA splicing (nuclear)
• prp42 U1 snRNA-associated protein required for pre-mRNA splicing and U1 snRNP biogenesis (nuclear)
• rlr1 Protein involved in transcription and transcription-associated recombination, has pleiotropic regulatory properties (nuclear)
• rse1 U2 snRNP-associated protein involved in pre-mRNA splicing, indirectly involved in ER to Golgi transport (nuclear)
• scp160 Protein involved in control of mitotic chromosome transmission (cytoplasmatic, endoplasmatic reticulum)
• sen1 SF1-type RNA helicase and positive effector of tRNA-splicing endonuclease required for intron cleavage for all 10 precursor tRNA families, involved in snRNA and snoRNA maturation (nuclear)
• sgv1 Serine/threonine protein kinase involved in pheromone adaptation pathway, cell cycle regulation, and regulation of transcription
• smd1 Spliceosomal snRNA-associated Sm core protein, also associated with telomerase TLC1 RNA (nuclear)
• smd2 Spliceosomal snRNA-associated Sm core protein, also likely associated with telomerase TLC1 RNA (nuclear)
• smd3 Spliceosomal snRNA-associated Sm core protein required for pre-mRNA splicing, also associated with telomerase TLC1 RNA (nuclear)
• snp1 U1 snRNA-associated protein with RNA recognition (RRM) domain (nuclear)
• snu71 U1 snRNA-associated protein (nuclear)
• sro9 Protein associated with ribosomes, has a potential role in translational accuracy (nuclear)
• srp1 Karyopherin-alpha or importin, involved in nuclear import, nuclear structure, chromosome segregation, and cell cycle (nuclear, cytoplasmatic, nuclear pore)
• sto1 Large subunit of the nuclear cap-binding protein complex (nuclear)
• tif4631 mRNA cap-binding protein (eIF4F) 150K subunit (nuclear)
• tif4632 mRNA cap-binding protein (eIF4F) 130K subunit (nuclear)
• ydl175c molecular function unknown
• ykl214c molecular function unknown

• luc7 U1 snRNA-associated protein (nuclear)
• mud1 U1 snRNP A protein (snRNA-associated protein) required for commitment complex formation during mRNA, contains two RNA recognition motif (RRM) domains.(nuclear)
• nam8 U1 snRNA-associated protein, essential for meiotic recombination, suppressor of mitochondrial splicing defects (nuclear)
• prp39 U1 snRNA-associated protein required for commitment of pre-mRNA to splicing pathway (nuclear)
• prp40 U1 snRNA-associated protein required for pre-mRNA splicing (nuclear)
• prp42 U1 snRNA-associated protein required for pre-mRNA splicing and U1 snRNP biogenesis (nuclear)
• smb1 Spliceosomal snRNA-associated Sm core protein, also likely associated with telomerase TLC1 RNA (nuclear)
• smd1 Spliceosomal snRNA-associated Sm core protein, also associated with telomerase TLC1 RNA (nuclear)
• smd2 Spliceosomal snRNA-associated Sm core protein, also likely associated with telomerase TLC1 RNA (nuclear)
• snp1 U1 snRNA-associated protein with RNA recognition (RRM) domain (nuclear)
• snu56 U1 snRNA-associated protein (nuclear)
• snu71 U1 snRNA-associated protein (nuclear)
• sto1 Large subunit of the nuclear cap-binding protein complex (nuclear)
• yhc1 U1 snRNP-specific C protein, component of the U1 snRNP that is needed for spliceosome assembly (nuclear)

• Ylr106c protein required for cell viability
• Yhr033w Hypothetical ORF
• Ygr090w protein required for cell viability
• Tom1 Protein required for the G2/M transition, has similarity to Rsp5p, a ubiquitin-protein ligase (E3 enzyme) (nuclear)
• Tfg1 RNA polymerase II transcription initiation factor TFIIF (factor g), 105 kDa subunit, component of RNA polymerase holoenzyme complex (nuclear)
• Spt5 Protein involved in chromatin structure that influences expression of many genes ( Pol II transcription elongation factor) (nuclear)
• Rpo21 RNA polymerase II, largest subunit (nuclear)
• Rpn1 Non-ATPase component of the 26S proteasome complex, required for degradation of proteins of the endoplasmic, also functions in RNA polymerase II transcription elongation (nuclear, endoplasmatic reticulum)
• Rpb3 RNA polymerase II, third-largest subunit (B45) (nuclear)
• Pup2 Proteasome subunit alpha5_sc (nuclear)
• Hxt6 High-affinity hexose transporter, member of the hexose transporter family of the major facilitator superfamily (plasma membrane, integral membrane)
• Hsp104 Heat shock protein required for induced thermotolerance and for resolubilizing aggregates of denatured proteins, important for the [psi-] to [PSI+] prion conversion (cochaperone) (nuclear, cytoplasmatic)
• Hef3 Translation elongation factor EF-3B, member of the non-transporter group of the ATP-binding cassette superfamily (cytoplasmatic)
• Cpr6 Cyclophilin (peptidylprolyl cis-trans isomerase or PPIase), interacts with Hsp82 (heat inducible chaperonin) (cytoplasmatic)
• Car2 Ornithine aminotransferase (ornithine oxo-acid aminotransferase), acts in the degradation of arginine, citrulline and ornithine (cytoplasmatic)
• Bcy1 Regulatory subunit of cAMP-dependent protein kinases (nuclear)
• ess1 Peptidylprolyl cis/trans isomerase, involved in cell cycle control, possibly involved in transcription termination or 3'-end processing of pre-mRNA

• Yjr072c required for cell viability
• RVB1 Putative 3' to 5' DNA/RNA helicase involved in ribosomal RNA processing (nuclear)
• Rpn7 Non-ATPase of the 26S proteasome complex that also functions in RNA polymerase II transcription elongation (nuclear)
• Rpn1Non-ATPase component of the 26S proteasome complex, required for degradation of proteins of the endoplasmic reticulum, also functions in RNA polymerase II transcription elongation (nuclear, endoplasmatic reticulum)
• Rnq1 unknown function
• Rgd1 GTPase-activating protein (GAP) for Rho3p and Rho4p
• Prb1 Protease B (yscB/PrB/cerevisin), serine protease of the subtilisin family with broad proteolytic specificity, important for protein degradation during sporulation (Lysosome/vacuole)
• Lys12 Homoisocitrate dehydrogenase, converts homoisocitrate to alpha-ketoadipate, the fourth step in the lysine biosynthesis pathway (Mitochondrial, cytoplasmatic)
• Idh1 Isocitrate dehydrogenase (NAD+) subunit 1, required for oxidative function of the tricarboxylic acid cycle (Mitochondrial)
• Bul1 Protein involved in the ubiquitination pathway (plasma membrane)
• Bck1 Serine/threonine protein kinase of the MEKK family involved in the cell wall integrity
• Arp2 Actin-related protein of the ARP2/3 complex involved in cell polarity and endocytosis (nuclear)
• Mkk2 MAP kinase kinase (MEK) serine/threonine protein kinase, involved in cell wall integrity pathway
• rsp5 Essential ubiquitin-protein ligase (E3 enzyme), a member of HECT domain family of ligases ( Cytoplasmic; Plasma membrane; Endosome/Endosomal vesicles; Peripheral membrane)

• Ylr392c Hypothetical ORF
• Ykr018c Hypothetical ORF
• Lsb1 Protein with weak similarity to chicken growth factor receptor- -binding protein, has an SH3 domain.
• Rpo26 Shared subunit of RNA polymerases I, II, and III (nuclear)
• Rpo21 DNA-directed RNA polymerase II, largest subunit (nuclear)
• Rpb3 RNA polymerase II, third-largest subunit (B45) (nuclear)
• Rpb5 25-kDa RNA polymerase subunit (common to polymerases

I, II and III) (nuclear)
• Rnq1 unknown function
• Pho84 High-affinity inorganic phosphate/H+ symporter, member of the phosphate:H[+] symporter (PHS) family of the major facilitator superfamily (MFS) (Plasma membrane)
• Hxt6 High-affinity hexose transporter, member of the hexose transporter family of the major facilitator superfamily (MFS) (Plasma membrane)
• Bul1 Protein involved in the ubiquitination pathway (Plasma membrane)
• rsp5 Essential ubiquitin-protein ligase (E3 enzyme), a member of HECT domain family of ligases ( Cytoplasmic; Plasma membrane; Endosome/Endosomal vesicles; Peripheral membrane)

Ubc13 Ubiquitin-conjugating (E2) enzyme involved in Rad6-dependent post-replicative repair pathway (nuclear, cytoplasmatic)

rsp5 Essential ubiquitin-protein ligase (E3 enzyme), a member of HECT domain family of ligases ( Cytoplasmic; Plasma membrane; Endosome/Endosomal vesicles; Peripheral membrane)

Mms2 Ubiquitin-conjugating enzyme variant (UEV) protein involved in resistance to DNA damaging agents

Bul1 Protein involved in the ubiquitination pathway (Plasma membrane)

• Pho84 High-affinity inorganic phosphate/H+ symporter, member of the phosphate:H[+] symporter (PHS) family of the major facilitator superfamily (MFS) (Plasma membrane)
• Dun1 Protein kinase required for induction of Rnr3 (Ribonucleotide reductase) and DNA repair genes after DNA damage
• Bul1 Protein involved in the ubiquitination pathway (Plasma membrane)
• rsp5 Essential ubiquitin-protein ligase (E3 enzyme), a member of HECT domain family of ligases ( Cytoplasmic; Plasma membrane; Endosome/Endosomal vesicles; Peripheral membrane)